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Image Search Results
Journal: Frontiers in Cellular Neuroscience
Article Title: Automated Live-Cell Imaging of Synapses in Rat and Human Neuronal Cultures
doi: 10.3389/fncel.2019.00467
Figure Lengend Snippet: Image acquisition. (A) In each well of a 96-well plate, a stack of 10 images separated by 1 μM in the z-axis was collected from five regions of interests (ROIs). The perfect focus system (PFS) uses an IR laser to locate the bottom of the plate so that image acquisition begins at a precise z-offset from the bottom of the well. Flow chart shows sequence of alignment, movement and acquisition (scan) for automated microscope controlled by the JOBS module in Elements software. (B) Rat hippocampal cultures in 96-well plates were transduced using a bicistronic HdAd virus with a hSyn promoter driving independent expression of postsynaptic density 95-enhanced green fluorescent protein (PSD95-eGFP) (green) and mCherry (red). Images were collected with laser scanning confocal microscopy or with wide-field microscopy as described in “Materials and Methods” section. Maximum intensity projections (MIPs; shown) for the confocal and wide-field (after deconvolution) images are uploaded for subsequent image processing. Scale bar: 10 μM.
Article Snippet: Images were acquired on a Nikon A1 confocal microscope (Nikon, Melville, NY, USA) using a 40 × (0.95 numerical aperture) air objective and controlled with the
Techniques: Sequencing, Microscopy, Software, Virus, Expressing, Confocal Microscopy